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2017-10-01 - Colloque/Présentation - poster - Anglais - 1 page(s)

Duprez Marie-Eve , Castro Cristiana , Cambier Joëlle, Devalckeneer Aude , Martin Thierry , Liénard Amandine, Vanderheyden Vincent, Saintenoy Yves, Hantson Anne-Lise , "Polycyclic aromatic hydrocarbons (PAHs) bioremediation of a contaminated soil. Isolation of native bacterial strains." in 10th World Congress of Chemical Engineering, Barcelone, Espagne, 2017

  • Codes CREF : Biochimie (DI3112), Biotechnologie (DI3800), Environnement et pollution (DI3840)
  • Unités de recherche UMONS : Biologie humaine et Toxicologie (M125), Géologie fondamentale et appliquée (F401), Génie des Procédés chimiques et biochimiques (F505)
  • Instituts UMONS : Institut des Sciences et du Management des Risques (Risques)
Texte intégral :

Abstract(s) :

(Anglais) Polycyclic aromatic hydrocarbons (PAHs) are non-polar organic compounds made up of two or more fused aromatic rings arranged in different structures (linear, angular or clustered). The sources of PAHs in environment may be anthropogenic or natural. The first ones include essentially the combustion of organic matters or fossil fuels (e.g. coal and wood burning, combustion engines, heat and power generation, tobacco smoke). The second ones are mainly volcanic eruptions and forest fires. Those compounds are thus widely dispersed in our environment in all natural compartments (air, water and soils). The US EPA has identified 16 PAHs as priority pollutants. Some of them are considered to have possible or probable human carcinogenic properties so that their environmental distribution and human exposure are of concerns. It is well-known that some micro-organisms such as bacteria and fungi are involved in the biodegradation of aromatic compounds. Those micro-organisms can either grow on a medium using PAHs as sole carbon and energy sources (mainly for bacteria) or they can produce enzymes such as lignine peroxidase (LiP), manganese peroxidase (MnP) or laccase (mainly for fungi and, more exactly white rot fungi) which have degradative properties on aromatic compounds. Those enzymes can catalyse one-electron oxidation of PAHs with formation of PAH quinones. [1, 2, 3] The main goal of the MEMORIS (MEthodologie de traitement et de MOnitoring pour la Réaffectation séquencée des sites Industriels Sévèrement Pollués) project (funded by the Région Wallonne (Pôle Greenwin, Duferco Wallonie and SITEREM), Belgium) is the combination of treatment and monitoring methods allowing sequential reuse of a severely polluted industrial site in order to decrease the financial impact of sanitation costs . One objective of the study is to build up a microbial consortium that will be used for significant degradation of PAHs content of the polluted site in presence of other pollutants (such as metallic compounds) that might influence the bioremediation process. The selected micro-organisms can be either local (isolated from soil and/or water samples) or laboratory commercial strains (Pseudomonas sp., Rhodococcus sp., Phanerochaete chrysosporium and Pleurotus ostreatus have been preselected). The influence of temperature on the microbial consortium will also be studied (the industrial soil at pilot scale will be heated). Isolated strains from water and/or soils samples, enzyme production of micro-organisms and first results of degradation tests using one or more isolated or commercial strains at laboratory scale (at least in liquid medium) will be presented.