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2017-03-30 - Colloque/Présentation - communication orale - Anglais - 17 page(s)

Gloire Mélanie, Villers Agnès , Colet Jean-Marie , Ris Laurence , "Reciprocal influence between APP expression and glucose metabolism in the hippocampus" in The 13th International conference on Alzheimer's and Parkinson's diseases , Vienne, Autriche, 2017

  • Codes CREF : Neurophysiologie (DI3224)
  • Unités de recherche UMONS : Neurosciences (M119)
  • Instituts UMONS : Institut des Sciences et Technologies de la Santé (Santé), Institut des Biosciences (Biosciences)
Texte intégral :

Abstract(s) :

(Anglais) The aim of this project is to better understand the link between APP expression and brain glucose metabolism and its impact on neuronal activity and synaptic connections in the hippocampus. Three levels of APP expression were investigated thanks to APP WT, HT and KO mice. To characterize APP roles in biochemical pathways we studied the correlation between APP expression level and metabolic profile in the hippocampus. The neurophysiological impact of genotype, glucose restriction and of the interaction between these two parameters was studied by extracellular electrophysiological recordings of cell excitability and synaptic activity in acute hippocampal slices incubated in control condition (10mM), mild (5mM) and severe glucose restriction (2.5mM). We also studied the effect of electrical activity disinhibition by adding the GABA-A receptor antagonist picrotoxin to the aCSF. 1HNMR spectroscopy highlighted several modifications in the metabolic profile according to the genotype. For example, APP KO mice showed an increase in the presence of glutamate while GABA was decreased. Electrophysiological recordings highlighted electricaldifferences in sensitivity to glucose restriction between WT, HT and KO mice and susceptibility to hyperexcitability in KO. Interestingly HT mice presented an intermediate phenotype in each performed experiments. We can already conclude that APP expression and glucose metabolism are indeed linked in the hippocampus and that further investigations need to be conducted to better understand this relationship.