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2007-03-13 - Article/Dans un journal avec peer-review - Anglais - 6 page(s)

Capron Brigitte , Wattiez Ruddy , Sindic Christian, Godaux Emile, Ris Laurence , "Tyrosine phosphorylation of rabphilin during long-lasting long-term potentiation" in Neuroscience Letters, 414, 3, 257-62

  • Edition : Elsevier, Limerick (Ireland)
  • Codes CREF : Biochimie (DI3112), Neurophysiologie (DI3224)
  • Unités de recherche UMONS : Neurosciences (M119), Biologie humaine et Toxicologie (M125), Protéomique et Microbiologie (S828)
Texte intégral :

Abstract(s) :

(Anglais) Long-term potentiation (LTP) is a persistent increase in the strength of synaptic transmission triggered by neuronal activity. Here, we submitted hippocampal slices to a perfusion of forskolin and IBMX, which induces a long-lasting LTP (>4 h) (L-LTP). We separated the proteins of the CA1 region by two-dimensional gel electrophoresis (2-DE). We then immunoblotted them using an anti-p-Tyr antibody. We found a protein whose tyrosine phosphorylation was unchanged 10 min after LTP induction but was dramatically increased after 1h, dropping back to its baseline after 4 h. This protein was identified as rabphilin using matrix-assisted laser desorption ionisation-time of flight mass spectrometry (MALDI-TOF MS). We also demonstrated that genistein, an inhibitor of tyrosine phosphorylation, prevented the development of the late phase of electrically-induced L-LTP. Our results suggest that rabphilin, a protein present in presynaptic terminals, could play a role in the late phase of L-LTP.

Notes :
  • (Anglais) Lecture en ligne: http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6T0G-4MP56C8-7-1&_cdi=4862&_user=532054&_pii=S0304394006013450&_origin=search&_coverDate=03%2F13%2F2007&_sk=995859996&view=c&wchp=dGLzVtz-zSkzS&md5=bb77e049a14c57415b964f6aa21a500d
  • (Anglais) Publié en ligne le 29 Decembre 2006
Identifiants :
  • DOI : 10.1016/j.neulet.2006.12.031
  • PMID : 17223267
  • ISSN : 0304-3940