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2017-05-22 - Colloque/Présentation - poster - Anglais - 1 page(s)

Duprez Marie-Eve , Castro Cristiana , Devalckeneer Aude , Martin Thierry , Liénard Amandine, Vanderheyden Vincent, Saintenoy Yves, Hantson Anne-Lise , "Treatment MEthodology and MOnitoring for sequenced Reallocation of severely polluted Industrial Sites (MEMORIS) project" in Green Chemistry and White Biotechnology 2017, Mons, Belgium, 2017

  • Codes CREF : Biochimie (DI3112), Biotechnologie (DI3800), Environnement et pollution (DI3840)
  • Unités de recherche UMONS : Biologie humaine et Toxicologie (M125), Géologie fondamentale et appliquée (F401), Génie des Procédés chimiques et biochimiques (F505)
  • Instituts UMONS : Institut des Sciences et du Management des Risques (Risques)
Texte intégral :

Abstract(s) :

(Anglais) 20th century was the golden age of steel industry in Wallonia, Belgium, especially in the provinces of Hainaut (cities of Charleroi or La Louvière), Walloon Brabant (Tubize) and Liège. The consequence of this past is the presence of multiple wastelands severely polluted by mainly PAHs (Polycyclic Aromatic Hydrocarbons), BTEX (Benzene, Toluene, Ethylbenzene, Xylene) and heavy metals. The reallocation of those sites for new activities is a major challenge and requires preliminary soil remediation to obtain pollution levels below legislation limits. The traditional technique used for soil remediation in Wallonia includes the excavation of contaminated soils, transport and treatment ex situ in specialised centres. However, this procedure is very expensive, may present health risks for local population and leads to negative carbon footprint. Consequently, in situ decontamination methods must be considered. In this context, the MEMORIS project (Treatment MEthodology and MOnitoring for sequenced Reallocation of severely polluted Industrial Sites) was born. The main goal of this project funded by the Walloon Region (Greenwin, Belgium), Duferco Wallonie and Siterem is the combination of treatment and monitoring methods allowing sequential reuse of a severely polluted industrial site in order to decrease the financial impact of sanitation costs. For this purpose, different techniques will be used in combination, namely, bioremediation (degradation of PAHs and BTEX), phytoremediation (phytostabilization of heavy metals), thermal treatment of the soil (stimulation of the microbial activity), monitoring (pollution evolution in continuous and during long periods) and health risk assessment (application of bio-indicators (invertebrates) and transposition of ecotoxicological tests to assess the impact of pollution on human health). The present work is mainly focused on bioremediation. It is well-known that some micro-organisms such as bacteria and fungi are involved in the biodegradation of aromatic compounds. Those micro-organisms can either grow on a medium using PAHs as sole carbon and energy sources (mainly for bacteria) or produce enzymes such as lignine peroxidase (LiP), manganese peroxidase (MnP) or laccase (mainly for fungi and, more exactly white rot fungi) which have degradative properties on aromatic compounds. One objective of the study is to build up a microbial consortium that will be used for significant degradation of PAHs content of the polluted site in presence of other pollutants (such as metallic compounds) that might influence the bioremediation process. The selected micro-organisms can be either local, isolated from soil and/or water samples, or laboratory commercial strains, namely Pseudomonas sp., Rhodococcus sp., Phanerochaete chrysosporium and Pleurotus ostreatus. The influence of temperature on the microbial consortium will also be studied, and the optimal value will be used at pilot scale for the industrial soil treatment. The global approach and first results will be presented.