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2017-10-24 - Colloque/Présentation - poster - Anglais - 1 page(s)

Delcourt Manon , Delsinne Virginie , Juszczak Florian , Colet Jean-Marie , Decleves Anne-Emilie , "Interest of 1H-NMR Based Metabonomics to Investigate Mitochondrial Bioenergetics Changes in a HFD-Mouse Model" in 8th world congress targeting mitochondria, Berlin, Allemange, 2017

  • Codes CREF : Toxicologie [toxines] (DI3236)
  • Unités de recherche UMONS : Biologie moléculaire (M122), Biologie humaine et Toxicologie (M125)
  • Instituts UMONS : Institut des Sciences et Technologies de la Santé (Santé)

Abstract(s) :

(Anglais) The growing epidemic of obesity particularly in the western world is considered a serious health and economic burden. Further understanding the underlying cellular pathways along with the metabolic changes involved in the development of obesity is of a great interest. To this respect, more and more evidences show that mitochondria are central and critical actors regarding the evolution of this disease at the cellular level (1–5). As an indicative tool of obesity-induced mitochondrial dysfunction, metabonomics appears as an unavoidable asset (6–8). Here, the aim was to study the mitochondrial dynamics, oxidative stress level and bioenergetics changes in an experimental mouse model of obesity. In this context, C57Bl/6 mice were randomly fed either a Low Fat Diet (LFD) or a HFD for 20 weeks. Twenty-four-hour urine collection were performed. After 20 weeks, urine samples were analyzed using 1H-NMR based metabonomics. Multivariate analysis of spectral data clearly underlines mitochondrial metabolome changes in the HFD group such as an increase of the Krebs cycle intermediates (succinate, alpha-ketoglutarate, citrate and fumarate). Those metabolic changes can be associated to changes in mitochondrial function and will be further analyzed with other mitochondrial structural and functional assays. In this purpose, histological (optic and electronic) analysis, immuno-histological analysis, RNA and protein determination are ongoing. In conclusion, metabonomics is an easy and rapid technique to detect changes in the mitochondrial function. In the context of a metabolic disorder such as obesity, combining omics-based approaches to structural and functional studies of mitochondria clearly seems to be a powerful tool.