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2006-08-20 - Article/Dans un journal avec peer-review - Anglais - 11 page(s)

Volcke C., Pirotton S., Grandfils Ch., Humbert C, Thiry P.A., Ydens I., Dubois Philippe , Raes M., "Influence of DNA condensation state on transfection efficiency in DNA/polymer complexes : an AFM and DLS comparative study" in Journal of Biotechnology, 125, 1, 11-21

  • Edition : Elsevier Science, Amsterdam (The Netherlands)
  • Codes CREF : Chimie macromoléculaire (DI1315), Catalyses hétérogène et homogène (DI1334)
  • Unités de recherche UMONS : Matériaux Polymères et Composites (S816)
  • Instituts UMONS : Institut de Recherche en Science et Ingénierie des Matériaux (Matériaux)
Texte intégral :

Abstract(s) :

(Anglais) Atomic force microscopy (AFM) is used to describe the formation process of polymer/DNA complexes. Two main objectives of this research are presented. The first one is to apply AFM as an effective tool to analyse DNA molecules and different polycation/DNA complexes in order to evaluate their degree of condensation (size and shape). The other one is to search for a relationship between the condensation state of DNA and its transfection efficiency. In this study, linear methacrylate based polymers and globular SuperFect polymers are used in order to induce DNA condensation. Ternary complexes, composed of methacrylate based polymers and polyethylene glycol (PEG)-based copolymers, are also investigated. AFM allows us to confirm good condensation conditions and relate them (or not) to transfection efficiencies. These AFM results (obtained after drying in air) are compared with measurements deduced from Dynamic Light Scattering (DLS) experiments performed in water. This comparison allowed us to identify the structural modifications resulting from deposition on the mica surface.

Notes :
  • (Anglais) Publié en ligne le 27 juin 2006
  • (Anglais) Lecture en ligne: http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6T3C-4K8S5F7-1-P&_cdi=4943&_user=532054&_pii=S0168165606001660&_origin=search&_coverDate=08%2F20%2F2006&_sk=998749998&view=c&wchp=dGLbVtb-zSkWA&md5=bd44070c436e7e5a2ad66a172eab7156
Identifiants :
  • DOI : 10.1016/j.jbiotec.2006.02.010