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2008-09-01 - Article/Dans un journal avec peer-review - Anglais - 8 page(s)

Saussez Sven , Decaestecker Christine, Mahillon Virginie, Cludts Stéphanie, Capouillez Aurélie, Chevalier Dominique, Kaltner Herbert, André Sabine, Toubeau Gérard, Leroy Xavier, Gabius Hans-Joachim, "Galectin-3 Upregulation during Tumor Progression in Head and Neck Cancers" in Laryngoscope, 118, 9

  • Edition : Lippincott Williams & Wilkins, Philadelphia (PA)
  • Codes CREF : Biochimie (DI3112), Histologie (DI3212), Biologie moléculaire (DI3111), Cancérologie (DI3349)
  • Unités de recherche UMONS : Anatomie et Biologie cellulaire (M112), Histologie (M118), Biologie moléculaire (M122)
Texte intégral :

Abstract(s) :

(Anglais) Objectives/Hypothesis: To examine the level of expression of galectin-3 in relation to neoplastic progression of hypopharyngeal squamous cell carcinomas (HSCCs) and laryngeal squamous cell carcinomas (LSCCs). Study Design: Retrospective study. Methods: Using a polyclonal antibody against galectin-3 without cross-reactivity to other galectins, we analyzed the presence of galectin-3 using quantitative immunohistochemistry in i) a series of 79 HSCCs compared with 16 normal epithelia, 20 low-grade dysplasia (Low_D) and 25 high-grade dysplasia (High_D) and in ii) a series of 58 LSCCs compared with 34 normal epithelia, 12 Low_D, and 18 High_D. In parallel, galectin-3 expression was studied using Western blotting on a series of 19 fresh biopsies from patients presenting a head and neck tumor. Results: Western blotting excluded a notable degree of proteolytic truncation of galectin-3 in situ. Immunohistochemical galectin-3 positivity expressed as percentage of cells was significantly higher in LSCCs and HSCCs than in Low_D (P = .01) or High_D (P = .0002), respectively. Increased expression of galectin-3 in HSCCs was accompanied by a shift from the cytoplasmic compartment to the nucleus (P = .007). In intertumor-type comparison, laryngeal carcinomas presented nuclear presence of galectin-3 only rarely (1 of 58 cases in laryngeal cancer vs. 27 of 79 cases in hypopharyngeal cancer, P = .00006) and a comparatively low labeling index (P <10-6). Conclusions: Our data reveal an association between level of presence of galectin-3 and neoplastic progression of HSCCs and LSCCs.

Identifiants :
  • ISSN : 0023-852X
  • DOI : 10.1097/MLG.0b013e31817b0718
  • PMID : 18677282