DI-UMONS : Dépôt institutionnel de l’université de Mons

Recherche transversale
(titres de publication, de périodique et noms de colloque inclus)
2017-09-11 - Colloque/Présentation - communication orale - Anglais - 1 page(s)

Trevisan Marie , Fossepre Mathieu , Ulrich S., Surin Mathieu , "Insight into the enzymatic activity on Nucleic acids-based supramolecular assemblies" in SysChem 2017, Sopron, Hongrie, 2017

  • Codes CREF : Chimie macromoléculaire (DI1315), Spectroscopie [électromagnétisme, optique, acoustique] (DI1255), Chimie analytique (DI1314)
  • Unités de recherche UMONS : Chimie des matériaux nouveaux (S817)
  • Instituts UMONS : Institut de Recherche en Science et Ingénierie des Matériaux (Matériaux), Institut des Biosciences (Biosciences)

Abstract(s) :

(Anglais) Enzyme-Responsive Materials (ERMs) have become an emerging research topic with potential applications in drug delivery, sensing and regenerative medicine1. Most ERMs studied so far were dealing with peptide-based materials, whereas those involving nucleic acids have been scarcely explored. In this context, we aim at studying the enzymatic activity towards supramolecular hybrids containing a functional π-conjugated molecule and an (oligo)nucleotide as substrate. . For this, we are taking advantage of the induced circular dichroism (ICD) signals observed for supramolecular assemblies in which (oligo)nucleotides are interacting with an achiral molecule2. These induced CD signals of supramolecular assemblies are extremely sensitive to the changes in the nucleic acids microenvironment, such as effects of DNA length and could therefore be used as a probe for enzymatic activity. The system selected for this study is an assembly of adenosine phosphate (AP) with Guanidinium-BisPyrene (GBP)3, to follow the ATP hydrolysis by an alkaline phosphatase. Our experiments show that the ICD signals of solutions composed of assemblies of GBP with AMP, or ADP, or ATP strongly differ at a given molar ratio (Fig. 1). Moreover, we observed that CD spectra of a mixture of two or three forms of AP are distinctive enough to be correlated to the proportion of the different AP present in solution. Our results suggest that the hydrolysis by alkaline phosphatase could be monitored by circular dichroism spectroscopy