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2018-05-15 - Colloque/Présentation - poster - Anglais - 1 page(s)

Sachdeva Neha , Leroy Baptiste , Poughon Laurent, Lasseur Christophe, Dussap Claude-Gilles, Wattiez Ruddy , "Increasing Oxygen Productivity of Arthrospira sp. PCC 8005 using alternative nitrogen sources: A bioengineering and proteomic outlook" in 1st joint AgroSpace-MELiSSA Workshop, Rome, Italy, 2018

  • Codes CREF : Biologie (DI3100)
  • Unités de recherche UMONS : Protéomie et Microbiologie (S828)
  • Instituts UMONS : Institut des Biosciences (Biosciences)
Texte intégral :

Abstract(s) :

(Anglais) The BIORAT-2 project aims at reaching a new step in the development of the MELiSSA Loop by making it self-sufficient in meeting the oxygen (O2) requirements and simultaneously increasing the degree of freedom, in terms of nitrogen (N) source use. Thus, in order to realize this objective and harness the potential of ammonium (NH4) as an alternative N source for the cultivation of Arthrospira sp. PCC 8005; its effect was evaluated (with respect to nitrate; NO3) on the O2 productivity, biochemical and proteomic profile of the cyanobacteria. Thus, a comprehensive meta-analysis (stoichiometric, proteomic and biochemical) was performed, to investigate the adaptation of Arthrospira sp. cells to fluctuating stream (transition) of alternative N sources (NH4 and NO3). The Photosim Model [1,2] was adapted to the BIORAT-2 conditions and used for the prediction of O2 productivity under the two N regimes. The present study, evaluated the effect of transition between the two N sources (NH4 and NO3 ) on O2 productivity, stoichiometric yields, metabolic, proteomic, biochemical profile of Arthrospira sp. PCC 8005 biomass. This study, thus not only focused on increasing the degree of freedom of the MELiSSA loop, but it also opened new avenues for the use of fluctuating stream of alternative N sources (from wastewater stream) for production of Arthrospira sp. PCC 8005, effectively bringing the MELiSSA technology as step closer towards embedding circular economies with photosynthetic biorefineries.